Taking IF images using Evos M5000 FL Microscope?

15 September 2023 0 9K Report

Hi,

I'm trying to take images for my fixed coverslips with cells. However, when I mount them using a mounting medium with DAPI, I try to take focused images. Few slides are better than the others in getting focused images, even though these slides were performed with the same protocol and similar reagents were used. Why?

My second question is If we want to take IF images to illustrate the abundance of two markers after differentiating our cells, How we can do so? What measures are needed when taking the images Diff vs. Undiff that are fixed onto two different slides/coverslips? For example, we fixed the light gain measures in Evos 5,000 when taking them. What else needs to be taken?

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