Grzegorz Wasilewski

4 Questions 2 Answers 0 Followers

Questions related from Grzegorz Wasilewski

How to increase glycolysis/glycolytic capacity in vitro while using Seahorse XF?

Dear all I am performing the mitostress seahorse assay in aortic endothelial cells. I am looking for a good positive control that would increase the glycolysis/glycolytic capacity when I treat the...

12 October 2022 4,317 1 View

What are best positive and negative controls for glucose uptake experiment?

Hello, I'm trying to determine the amount of glucose consumption using 14C-labelled 2-DG. What would be the good controls to use in the experiment? For negative I planned to use low glucose...

24 August 2022 5,194 2 View

Is radioactive calcium-45 uptake mostly in bone or also in the other tissues?

I am planning to feed rats with radioactive calcium. Is there any evidence that the radioactive uptake prefers the bone sites of the body in comparison to vasculature ?

21 June 2017 5,353 3 View

What is the optimum percentage of SDS-PAGE for proteins of mass 34kDa and 175kDa when both need to be loaded on one gel ?

I am trying to detect proteins using Western Blot Technique. Due to the size difference, smaller protein of 36kDa was not seen on the blot read-out. Would gradient gel be a better idea to detect...

05 January 2016 5,715 9 View