Grzegorz Wasilewski

9 Questions 10 Answers 0 Followers

Questions related from Grzegorz Wasilewski

How to increase glycolysis/glycolytic capacity in vitro while using Seahorse XF?

Dear all I am performing the mitostress seahorse assay in aortic endothelial cells. I am looking for a good positive control that would increase the glycolysis/glycolytic capacity when I treat the...

12 October 2022 4,392 1 View

What are best positive and negative controls for glucose uptake experiment?

Hello, I'm trying to determine the amount of glucose consumption using 14C-labelled 2-DG. What would be the good controls to use in the experiment? For negative I planned to use low glucose...

24 August 2022 5,262 2 View

Why is calcium not part of osteogenic differentiation medium of mesenchymal stem cells?

Hi Osteogenic differentiation media is usually composed of B-glycero-phopshate, ascorbic acid and dexamethasone. Why is calcium not used to promote hydroxyapatite formation in vitro ?

04 February 2020 7,112 4 View

What is the best media to differentiate mesenchymal stem cells towards smooth muscle cell phenotype?

Literature provides variety of protocol to differentiate smooth muscle cells from MSC's. 1. What differences could I expect between alpha-MEM/DMEM-F12 both with either L-glutamine or Glutamax?...

19 February 2019 3,816 2 View

Is radioactive calcium-45 uptake mostly in bone or also in the other tissues?

I am planning to feed rats with radioactive calcium. Is there any evidence that the radioactive uptake prefers the bone sites of the body in comparison to vasculature ?

21 June 2017 5,402 3 View

I am planning to use CT/PET scanners to track the localisation and deposition of calcium over time in Sprague-Dawley rats

What is the best method to do it? Rats will be fed with high calcium diet. I want to measure where the calcium is deposited over time Radioactive Calcium 45, 19F? I am really lost about the best...

20 June 2017 6,915 12 View

How many smooth muscle cells should I seed per well in 48-well plate along with alginate gell encapsulation?

Thanks!

22 March 2017 4,314 0 View

Has anyone grown VSMC's (vascular smooth muscle cells) in alginate?

If so what did you do in terms of assays?

20 March 2017 5,746 0 View

What is the optimum percentage of SDS-PAGE for proteins of mass 34kDa and 175kDa when both need to be loaded on one gel ?

I am trying to detect proteins using Western Blot Technique. Due to the size difference, smaller protein of 36kDa was not seen on the blot read-out. Would gradient gel be a better idea to detect...

05 January 2016 5,790 9 View