Gonzalo Sanchez

6 Questions 5 Answers 0 Followers

Questions related from Gonzalo Sanchez

Chloride Replacement in External Buffer, what is best alternative for NaCl?

Hi! I would be grateful to get some feedback about replacing NaCl with a different salt to avoid chloride in the recording buffer (I aim at performing live imaging in a chloride free solution). I...

31 October 2023 871 0 View

PiP2 effect on TRP channels?

Hi, I am wondering whether there is any extensive list of actions of Phosphatidylinositol 4,5-bisphosphate (PiP2) on Transient Receptor Potential (TRP) channels. As far I as understand it, PiP2...

19 September 2017 9,491 6 View

TRP channels blockade

Hi! I am trying to find the best way to block transient receptor potential channels (TRP channels). Could anyone suggest a blocker targeting the largest number of subtypes? Lots of thanks

02 August 2017 5,869 3 View

Can I define dynamic ROIs?

Hi! Does anyone know if it is possible to define an ROI that changes its shape in an image sequence? I am using ImageJ software and got to the point of having the edge of the shape established but...

10 January 2017 9,215 1 View

Which is the best antibody to label the cilium?

I would like to tag pancreatic beta cell's cilia, as far as I know adenylyl cyclase III is a good marker among a few others. Are there any commercially available antibodies with validated...

04 September 2016 9,606 3 View

How could I block Ca2+ permeable AMPA-R?

Has anyone used Naspm, (N-[3-[4-(3-aminopropylamino)butylamino]propyl]-2-naphthalen-1-ylacetamide;trihydrochloride) a calcium-permeable AMPA-receptors blocker, in the pipette internal...

19 July 2016 6,699 3 View