Giulia Verrilli

7 Questions 3 Answers 0 Followers

Questions related from Giulia Verrilli

Is it important to pre-treat the cells with unlabelled oxLDL before adding the labeled probe Dil-oxLDL?

Hello to everyone. I'd like some advice on visualising foam cells by FACS. The invitrogen protocol suggests pretreating macrophages with unlabelled ox-LDL to occlude the LDL receptors before...

14 August 2024 464 0 View

Staining of foam cells derived from PBMC differentiated to macrophages. Does anyone have experience with this type of assay?

Hi everyone, I am trying to induce foam cells in macrophage differentiated from PBMC. Which type of staining do you prefer to use? Nile Red or Oil RED O ? What concentration and timing of ox-LDL...

02 July 2024 8,342 0 View

Which negative control do you recommend for In vitro Foam Cell Formation Assay?

Hi everyone I have to perform the Foam Cell Assay with OIL RED O staining. Can you recommend a compound that inhibits LDL oxidation? Also, can anyone recommend oxLDL to use?

15 May 2024 6,436 1 View

What do you think I'm doing wrong?

Hi everyone, I differentiate THP-1 in macrophages with PMA 48h + 24h rest. After treatment with LPS I would expect an increase in ROS. But this increase compared to control I cannot get. I have...

26 March 2024 4,819 6 View

When does the Griess colorimetric assay produce yellow instead of pink?

I am using the Nitrate/Nitrite Colorimetric Assay Kit from Cayman. When I used the nitrate+nitrite determination assay with nitrate reductase on the same samples' supernatant, it turned pink. When...

19 February 2024 3,365 0 View

What medium can I use (instead of RPMI) on differentiated THP1 if my goal is to measure small changes in nitrate levels?

I differentiated THP1 cells into macrophages. Subsequently, macrophages will be treated with substances. Next, the Griess Reagent test will be used to determine nitrate levels. The Griess kit...

25 January 2024 9,579 1 View

How can I dissolve Nitrate Reductase ≥300 U/g and use it at 300 U/L?

I would like to performe the GRIESS reagent kit from biotium. The nitrate reductase enzyme (Sigma catalog no. N7265) has an activity ≥300 U/g. By protocol I am to use it at a final concentration...

22 July 2023 1,199 3 View