Genesis Marrero

5 Questions 20 Answers 0 Followers

Questions related from Genesis Marrero

What could explain salt crystal formation on the surface of samples with cells cultured on calcium phosphate discs?

Hello all, We cultured MSCs on calcium phosphate discs for 3 days and 7 days. We are seeing strange crystal-like precipitates or something of the sort (images attached). They are found wherever...

25 July 2023 3,873 4 View

What might explain the EDS results showing greater wt% of Mg in undoped "pure" beta-TCP powder vs doped beta-TCP?

Hi All, We are trying to add Magnesium & Strontium dopants to our Beta Tricalcium Phosphate (b-TCP) powder. Recently, we obtained EDS data on the undoped b-TCP and doped b-TCP to see if the...

16 November 2022 4,401 3 View

How to troubleshoot fungal contamination in mammalian cell culture?

Hi all, I am dealing with a very stubborn fungal contamination issue in my mammalian cell cultures. I could really use some pointers for how to get rid of the contamination at present and how to...

30 July 2022 3,968 8 View

Methods to Detach & Lyse Cells Cultured on 3D-Printed Hydroxyapatite Scaffolds?

I am culturing MSCs on 3D-printed hydroxyapatite scaffolds. We need to detach cells from the 3D to analyze/quantify overall DNA content using Quant-iT PicoGreen dsDNA Reagents and Kit. However,...

21 June 2022 5,702 2 View

Why are my NIH 3T3 Cells dying after flow cytometry? Do they need CO2 levels controlled when running flow cytometry (loss of ~40% viability)?

UPDATE: the issue was the flow pressure being too high and cells being too sensitive to a loss of membrane integrity. I lowered the flow pressure and used a reagent/buffer that is designed to...

01 October 2021 1,344 2 View