Genesis Marrero

8 Questions 26 Answers 0 Followers

Questions related from Genesis Marrero

Can SBF (Simulated Body Fluid) be sterilized in an autoclave or does it need to be sterile filtered?

After formulation, can SBF be sterilized in the autoclaved without damaging the components? Or should it be sterile-filtered?

12 February 2024 5,387 1 View

TaqMan vs Traditional qRT-PCR?

Is It more cost effective to analyze multiple genes using TaqMan versus traditional qRT-PCR? What other main advantages and disadvantages exist for each method? I’m trying to analyze several...

06 February 2024 4,434 4 View

What is the correct way to calculate the optimal cell seeding density for a porous 3D-printed scaffold?

What is the correct way to calculate the optimal initial cell seeding density for a porous 3D-printed scaffold? The 3D-printed scaffolds are gyroid cubes (1cm^3), with ~78% porosity, a...

06 February 2024 4,149 0 View

What could explain salt crystal formation on the surface of samples with cells cultured on calcium phosphate discs?

Hello all, We cultured MSCs on calcium phosphate discs for 3 days and 7 days. We are seeing strange crystal-like precipitates or something of the sort (images attached). They are found wherever...

25 July 2023 3,882 4 View

What might explain the EDS results showing greater wt% of Mg in undoped "pure" beta-TCP powder vs doped beta-TCP?

Hi All, We are trying to add Magnesium & Strontium dopants to our Beta Tricalcium Phosphate (b-TCP) powder. Recently, we obtained EDS data on the undoped b-TCP and doped b-TCP to see if the...

16 November 2022 4,406 3 View

How to troubleshoot fungal contamination in mammalian cell culture?

Hi all, I am dealing with a very stubborn fungal contamination issue in my mammalian cell cultures. I could really use some pointers for how to get rid of the contamination at present and how to...

30 July 2022 3,979 8 View

Methods to Detach & Lyse Cells Cultured on 3D-Printed Hydroxyapatite Scaffolds?

I am culturing MSCs on 3D-printed hydroxyapatite scaffolds. We need to detach cells from the 3D to analyze/quantify overall DNA content using Quant-iT PicoGreen dsDNA Reagents and Kit. However,...

21 June 2022 5,710 2 View

Why are my NIH 3T3 Cells dying after flow cytometry? Do they need CO2 levels controlled when running flow cytometry (loss of ~40% viability)?

UPDATE: the issue was the flow pressure being too high and cells being too sensitive to a loss of membrane integrity. I lowered the flow pressure and used a reagent/buffer that is designed to...

01 October 2021 1,352 2 View