8 Questions 18 Answers 0 Followers
Questions related from Gan Jen Yang
Hi, Anyone have idea that what is the species of mold that grow on the timber pieces (please refer to attached photo Thanks
07 July 2016 5,890 7 View
I am trying to add sulfamic acid and calcium hypochlorite into my 0.1M citrate buffer. The buffer was prepared at pH 5 and I expect that the pH will not fluctuate much after addition of these...
04 April 2015 9,358 4 View
I would like to dissolve calcium hypochlorite with water or acid buffer, but calcium hypochlorite will settle down after the solution was left aside for a short while. Is there any way to maintain...
03 March 2015 2,125 11 View
I am trying to develop a method to stain suspension ES cells, which will be later quantified using flow cytometry. However, the result I get from flow cytometry shows that only a small population...
02 February 2014 468 2 View
I have primer dimerization when I run PCR on my samples but I don't have any primer-dimer when I carried out optimization by using my plasmid. I use 0.20ng of DNA or plasmid, 0.5 uL of 10pmol...
05 May 2013 8,483 12 View
Can someone recommend any kit/methods, which can easily isolate DNA from embryos for the purpose of PCR.
04 April 2013 1,698 9 View
I am transfecting my zebrafish ES cell line with a GFP tag plasmid. Besides counting GFP cell by using hemocytometer, is there any way to calculate the transfection efficiency?
03 March 2012 4,437 9 View
Will the transfected cells loss their plasmid after they are subcultured and split into low cell density?
02 February 2012 9,708 3 View
