8 Questions 18 Answers 0 Followers
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Hi, Anyone have idea that what is the species of mold that grow on the timber pieces (please refer to attached photo Thanks
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I am trying to add sulfamic acid and calcium hypochlorite into my 0.1M citrate buffer. The buffer was prepared at pH 5 and I expect that the pH will not fluctuate much after addition of these...
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I have primer dimerization when I run PCR on my samples but I don't have any primer-dimer when I carried out optimization by using my plasmid. I use 0.20ng of DNA or plasmid, 0.5 uL of 10pmol...
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I am transfecting my zebrafish ES cell line with a GFP tag plasmid. Besides counting GFP cell by using hemocytometer, is there any way to calculate the transfection efficiency?
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Will the transfected cells loss their plasmid after they are subcultured and split into low cell density?
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