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Questions related from Gan Jen Yang
Hi, I am running a titration of QAC with sodium tetraphylborate using autotitrator from Metrohm. The titrator identifies multiple end point with different ERC value. Would like to know which EP...
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I am trying to add sulfamic acid and calcium hypochlorite into my 0.1M citrate buffer. The buffer was prepared at pH 5 and I expect that the pH will not fluctuate much after addition of these...
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Comparing homologous recombination and zinc finger nuclease, which method will be able to give better knockout efficiency in embryonic stem cells?
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I am trying to develop a method to stain suspension ES cells, which will be later quantified using flow cytometry. However, the result I get from flow cytometry shows that only a small population...
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I am facing problems in raising up my zebrafish larvae, most of them start to die after 1week post fertilization. I get many different opinions regarding foods should be fed, some said they can be...
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I am transfecting my zebrafish ES cell line with a GFP tag plasmid. Besides counting GFP cell by using hemocytometer, is there any way to calculate the transfection efficiency?
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