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Questions related from Gan Jen Yang
Hi, I am running a titration of QAC with sodium tetraphylborate using autotitrator from Metrohm. The titrator identifies multiple end point with different ERC value. Would like to know which EP...
18 April 2024 770 1 View
Hi, Anyone have experience in identify the sapwood and heartwood of Hem-Fir and SPF sawn timber by using dye? I found that bromocresol green and bromophenol blue work well on these 2 species but...
06 January 2017 7,723 3 View
I added hypochlorous acid (prepared and filtered from calcium hypochlorite solution) into a citrate buffer (mixture of 3M citric acid and 3M sodium citrate, ratio 40:60). I found that a layer a...
19 May 2015 6,039 6 View
I am trying to add sulfamic acid and calcium hypochlorite into my 0.1M citrate buffer. The buffer was prepared at pH 5 and I expect that the pH will not fluctuate much after addition of these...
09 April 2015 7,773 3 View
Hi guys, After I mixed basic copper carbonate with sorbitol, water and NaOH, precipitation of sludge was found. I was told that by adding monoethanolamine may help to dissolve the sludge. Besides...
28 August 2014 9,824 5 View
Comparing homologous recombination and zinc finger nuclease, which method will be able to give better knockout efficiency in embryonic stem cells?
29 April 2014 2,138 2 View
I am trying to develop a method to stain suspension ES cells, which will be later quantified using flow cytometry. However, the result I get from flow cytometry shows that only a small population...
21 February 2014 9,050 2 View
Besides massaging their belles and breeding them more often, are there any alternative methods to treat them?
30 January 2013 3,140 1 View
What is the best density of raising juvenile fish? Because I found that my fish appeared to be inactive, low feeding on food when their density are low (about 6 fish per 1L water). Some said the...
28 October 2012 2,602 7 View
I am facing problems in raising up my zebrafish larvae, most of them start to die after 1week post fertilization. I get many different opinions regarding foods should be fed, some said they can be...
08 September 2012 3,116 24 View
I am transfecting my zebrafish ES cell line with a GFP tag plasmid. Besides counting GFP cell by using hemocytometer, is there any way to calculate the transfection efficiency?
27 March 2012 273 8 View