Fawaz Alassaf

6 Questions 11 Answers 0 Followers

Questions related from Fawaz Alassaf

Does the decrease in the TEER value (always) associated with an increase in the flux of FD-4 across endothelial cell monolayers?

I am investigating the permeability of Human Brain Microvascular Endothelial Cells for certain compounds with TEER, and a functional assay with FITC-Dextran. I've got a decrease in TEER values for...

18 April 2017 3,804 5 View

Do the cells have to be serum-starved for measuring Integrity, Permeability?

I am trying to measure the permeability of Human Brain Microvascular Endothelial Cells against certain compounds with TEER, functional assay with FITC-Dextran and checking the expressing of...

02 November 2016 430 3 View

Do I really need a permeabilisation step in VE-Cadherin immunocytochemistry?

I am investigating VE-Cadherin expression in Human Coronary Artery Endothelial Cells towards certain compounds. VE-Cadherin is a membrane protein. I got (attached picture) VE-Cadherin expression...

18 March 2016 7,898 7 View

How do I assess Cadherin in porcine coronary artery?

I would like to investigate the effect of certain compounds on Cadherin in blood vessels. Basically, I am doing experiments on porcine coronary arteries. I am willing to know whether my...

23 January 2016 329 3 View

Does anyone have any information/methods on measuring vascular permeability in porcine isolated coronary arteries (Not cell line culture)?

I am trying to examine the vascular permeability of certain compound. I think this compound may have an effect on vascular permeability. I am working on porcine isolated coronary arteries. My...

28 January 2015 5,976 3 View

Do antihistamine drugs increase or decrease total IgE?

I am aware that blood IgE levels can be reduced by (always) treating what is the cause behind raised IgE levels, but, can plasma concentrations be markedly affected (decreased) in...

01 January 1970 1,234 3 View