8 Questions 9 Answers 0 Followers
Questions related from Emre Can Tüysüz
I have mRNA microarray data analysis from both Affymetrix (Hu Gene 2.1 microarray) and Agilent (Human Gene Expression 4×44 K Microarray Kit (Design ID 014850, Agilent Technologies). Is it...
14 February 2018 2,823 3 View
I am using Mouse (ICR) Inactivated Embryonic Fibroblasts (Thermofisher, Cat No:A24903) to culture ES-E14TG2a Cell Line ( Sigma, Cat No: 08021401-1VL). It is suggested that approximately 1.5*10^6...
06 May 2017 7,494 5 View
FBS includes various protease inhibitors paticularly α1-antitrypsin and can inactivate trypsin. Can FBS also inactivate accutase?
26 March 2016 9,657 1 View
I used Trizol Reagent to isolate RNA. I did what the protocol says. Furthermore, I used 10 ug Glycogen during the RNA precipitation step because of a small number of cells. After ethanol washing,...
23 October 2014 3,615 3 View
I isolate total RNA from approximately 10k-15k cell through Trizol reagent. However, the purity of RNA sample is very low. Can you recommend a kit for total RNA isolation from small quantities sample?
10 April 2014 9,751 7 View
I used an X-treme siRNA transfection reagent to transfect miRNA. After that I performed Real-Time PCR to validate my transfection. I do my transfection at 2 different times (8 hour and 72 hour)....
02 December 2013 3,844 2 View
I have prepared cell culture from glioblastoma tissue however I should show that this cell culture is a glioblastoma cell culture. How I can prove it? What is the best marker gene that is...
07 November 2013 8,129 2 View
In my experiment, I will use a one type of human Glioblastoma cell line for a positive control. I have U-87 Gbm cell line but U-87 cell line has astrocytoma disease, too. Can I use it? Or which...
01 November 2013 3,371 15 View
