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I have mRNA microarray data analysis from both Affymetrix (Hu Gene 2.1 microarray) and Agilent (Human Gene Expression 4×44 K Microarray Kit (Design ID 014850, Agilent Technologies). Is it...
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I am using Mouse (ICR) Inactivated Embryonic Fibroblasts (Thermofisher, Cat No:A24903) to culture ES-E14TG2a Cell Line ( Sigma, Cat No: 08021401-1VL). It is suggested that approximately 1.5*10^6...
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FBS includes various protease inhibitors paticularly α1-antitrypsin and can inactivate trypsin. Can FBS also inactivate accutase?
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I used Trizol Reagent to isolate RNA. I did what the protocol says. Furthermore, I used 10 ug Glycogen during the RNA precipitation step because of a small number of cells. After ethanol washing,...
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I isolate total RNA from approximately 10k-15k cell through Trizol reagent. However, the purity of RNA sample is very low. Can you recommend a kit for total RNA isolation from small quantities sample?
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I used an X-treme siRNA transfection reagent to transfect miRNA. After that I performed Real-Time PCR to validate my transfection. I do my transfection at 2 different times (8 hour and 72 hour)....
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I have prepared cell culture from glioblastoma tissue however I should show that this cell culture is a glioblastoma cell culture. How I can prove it? What is the best marker gene that is...
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In my experiment, I will use a one type of human Glioblastoma cell line for a positive control. I have U-87 Gbm cell line but U-87 cell line has astrocytoma disease, too. Can I use it? Or which...
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