Emre Can Tüysüz

12 Questions 12 Answers 0 Followers

Questions related from Emre Can Tüysüz

Is it possible to analyze mRNA microarray data provided from both Affymetrix and Agilent in Genespring?

I have mRNA microarray data analysis from both Affymetrix (Hu Gene 2.1 microarray) and Agilent (Human Gene Expression 4×44 K Microarray Kit (Design ID 014850, Agilent Technologies). Is it...

14 February 2018 2,864 3 View

Is High MEF density a problem during mESC culturing?

I am using Mouse (ICR) Inactivated Embryonic Fibroblasts (Thermofisher, Cat No:A24903) to culture ES-E14TG2a Cell Line ( Sigma, Cat No: 08021401-1VL). It is suggested that approximately 1.5*10^6...

06 May 2017 7,540 5 View

How can I analyze miRNA Microarray result in Genespring Program?

We have done miRNA Microarray using Agilent Human miRNA Microarray KitVer. 3.0 (Cat No: AGT-G4470C). I have .gpr files of my samples but I could not analyze their miRNA profile on genespring. How...

26 January 2017 242 1 View

Can FBS inactivate Accutase?

FBS includes various protease inhibitors paticularly α1-antitrypsin and can inactivate trypsin. Can FBS also inactivate accutase?

26 March 2016 9,716 1 View

What is an appropriate medium for freezing human astrocytes?

We have normal human astrocytes from lonza(cat no:cc-2565). We want to freeze our cells for storage but there is no instruction in spesification sheet for freezing. What is appropriate medium for...

12 March 2015 338 2 View

What should I do if my RNA pellet did not dissolved in RNase free water?

I used Trizol Reagent to isolate RNA. I did what the protocol says. Furthermore, I used 10 ug Glycogen during the RNA precipitation step because of a small number of cells. After ethanol washing,...

23 October 2014 3,684 3 View

Why can I not detect CD133 gene expression from CD133+ subpopulation of cancer cells?

I sorted CD133+ cells from my population. I isolate RNA through Trizol from these populations to evaluate gene expression levels. I synthesized cDNA through QuantiTect Reverse Transcription Kit. I...

09 September 2014 2,757 4 View

Can anyone help with RNA isolation from a small quantities sample?

I isolate total RNA from approximately 10k-15k cell through Trizol reagent. However, the purity of RNA sample is very low. Can you recommend a kit for total RNA isolation from small quantities sample?

10 April 2014 9,810 7 View

Can anyone help with transfection confirmation?

I used an X-treme siRNA transfection reagent to transfect miRNA. After that I performed Real-Time PCR to validate my transfection. I do my transfection at 2 different times (8 hour and 72 hour)....

02 December 2013 3,884 2 View

Why is S-warfarin is more potent than R-Warfarin?

Is Vitamin K the reason?

21 November 2013 6,423 2 View

What is the best diagnostic tool for glioblastoma?

I have prepared cell culture from glioblastoma tissue however I should show that this cell culture is a glioblastoma cell culture. How I can prove it? What is the best marker gene that is...

07 November 2013 8,174 2 View

Which human glioblastoma cell line is appropriate for positive control?

In my experiment, I will use a one type of human Glioblastoma cell line for a positive control. I have U-87 Gbm cell line but U-87 cell line has astrocytoma disease, too. Can I use it? Or which...

01 November 2013 3,419 15 View