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Questions related from Dumindu Kavinda
Hello everyone, I would like to know whether we can use the vibratome to section zebrafish larvae (8-9 days post fertilization) for histology. We have a Leica VT1200S vibratome in our lab....
16 April 2024 2,029 0 View
Hi, May I know the recommended blocking buffers used in zebrafish immunofluorescence staining? In one protocol it advices to use 3% BSA, 1% Triton X-100 and 1% DMSO in PBST (0.1% Tween 20 in PBS)...
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Hello everyone, I would like to know why LX2 cells produce a large number of lipid droplets during passages and they show a activated hepatic stellate cell morphology. Here is the procedure I...
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Hello everyone, I'm trying to extract proteins from J774A1 cells after treating them with 200ng/mL LPS for 4 hours followed by 5mM ATP treatment for another 4 hours to study inflammasome...
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I would like to know what would be the ideal time to extract nuclear proteins from RAW 264.7 macrophages after they are treated with LPS.
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