Dmytro Nesterenko

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Questions related from Dmytro Nesterenko

Did anyone experience low frequency stimulation inducing potentiation, instead of depression in LFP recordings from accute hippocampal slice?

Hello! I noticed that in some of my recordings, 15m of 1Hz stimulation result in increase of EPSP slope and amplitude, instead of decrease, like you would expect. For example in this figure. It...

13 March 2025 3,454 1 View

In ephys recording chamber, that uses a pump to flow in and remove ACSF, how would you call the outflow tube/needle used to avoid pulsation of fluid?

It looks like a syringe needle with a flat diagonal opening, or a blunt tube with slits cut on the sides, and it's used to suck out the ACSF and keep the fluid level stable in the recording...

23 October 2023 9,488 3 View

When recording fEPSP input/output curve in hippocampal slices, sometimes further increasing stimulation amplitude reduces output EPSP. Why?

I understand increasing stimulation causing stronger EPSP, but sometimes, after certain point, further increasing stimulation only decreases EPSP. What is the mechanism of this? For example...

26 May 2023 7,866 4 View

Dmytro Nesterenko

Did anyone experience low frequency stimulation inducing potentiation, instead of depression in LFP recordings from accute hippocampal slice?

In ephys recording chamber, that uses a pump to flow in and remove ACSF, how would you call the outflow tube/needle used to avoid pulsation of fluid?

When recording fEPSP input/output curve in hippocampal slices, sometimes further increasing stimulation amplitude *reduces* output EPSP. Why?

When recording fEPSP input/output curve in hippocampal slices, sometimes further increasing stimulation amplitude reduces output EPSP. Why?