I am trying to determine kinetic constants for my reaction. I have the enzyme activity in IU/ml. The unit of activity indicates one micromole of product formed per unit time. If we have to plot v vs S and calculate vmax and km, then do we plot enzyme activity vs Substrate conc (in micromoles)???
Estimado amigo: Primero, Usted Dębe Obtener VELOCIDADES Iniciales (Vo (micromoles min'1)), Una Diferentes concentrations de sustrato. Es Decir,: Del Gráficos C(E) (micromoles) vs t (min), A different concentrations de sustrato, se pueden Calcular Diferentes VELOCIDADES Iniciales (Vo). Finalmente, Obtener Una gráfica de Vo vs. Csubstrate.
Espero Que AYUDE.
A Unit of of enzyme activity may not have much meaning in terms of the kinetic constants Km and Vmax. The Unit is defined as the amount of enzyme required to produced a certain amount of product in a certain amount of time under specified conditions. This definition does not require that this amount of product be formed under initial rate conditions, which is a requirement for the usual method of measuring kinetic constants. Moreover, the Unit measurement is made at a single substrate concentration, whereas the usual method of measuring kinetic constants requires that measurements be made at several substrate concentrations.
Adam Shapiro is perfectly correct and provides a straightforward answer to your question. If you need rate data for calculation of kinetic parameters, determine initial rates of reaction by measuring the concentration of product formed per unit time. Confine measurements to the early stages of reaction to be sure that the reactio rate is linear with time
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