Daeseong Kim

3 Questions 2 Answers 0 Followers

Questions related from Daeseong Kim

How to extract native protein from mammalian cell?

Hello, Currently, I am conducting an experiment using a reporter cell (a mammalian cell expressing target protein in cytosol). The experiment is not going well. So I would like to check whether...

07 September 2022 4,398 3 View

Is there any reference about correlation between protein folding energy and its expression level?

I am currently studying how to introduce mutations to improve the expression level of proteins. My lab has a system that can model the structure of proteins through alphafold, which models mutants...

11 October 2021 349 2 View

How to add restriction enzyme site to given peptide sequence for molecular cloning?

I am currently conducting molecular cloning. I want to put a new restriction enzyme site in a given peptide sequence, and I want to preserve the peptide sequence as much as possible. More...

12 April 2021 1,099 3 View