I decide to perform PCR on a non-natural nucleotide strand. The strand is composed of one modified region(40 non-natural nucleotides) and two primer sites(20 nts natural DNA for each). The modification would be either 2-FANA, 2-O-methyl or 2-amine(all on 2' position on the sugar ring). Dose anyone have experience with that or any suggestion on the polymerase I should try first? Thanks for concerning!