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Questions related from Caroline o carroll
I want to coat 12 well inserts with collagen I Bovine from gibco. I am just wondering how would you dilute to achieve a concentration of 5ug/cm2 if the starting concentration is 5mg/ml and the...
23 November 2017 6,860 1 View
I plate 50ul of bacteria OD 0.5-0.7 diluted to 10-7 in a sterile 96 well plate. I then added 40ul of media/seaweed extracts varying concentrations. I incubate at 37oC for 24 hours and after 24hrs...
03 March 2017 7,500 4 View
I am going to be culturing streptococci bacteria in the lab. I know the suggested plates to grow them on are blood agar but i know this are difficult to make. I am just wondering is there another...
20 January 2017 10,033 3 View
I can only find blank sterile disc at 10mm in diameter and its suggested to use 6mm discs. Would it make a negative impact on my results if i used the 10mm disc.
20 January 2017 5,534 0 View
I am going to be growing the three mention strains and just wondering if trypticase soy yeast extract medium be ok for all three strains or do i need to grow them on more selective media ?
19 December 2016 5,087 5 View
I am relatively new to cell culturing. I need to co - culturing MAC-T cell with bacteria. If anyone would have a protocol or any papers that might be relevant , I would greatly appreciate it .
16 December 2016 9,484 2 View
what Insulin would you add to DMEM media if your trying to culture MAC-T cell line. Its my first time trying to culture MAC-T cell line and I am not 100% sure about the insulin.
14 November 2016 6,559 4 View
