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Questions related from Aziz Ul Ikram
Seeds obtained from heterozygous T0 mutants obtained through CRISPR CAS9 were sown and the plants raised were properly genotyped. Following normal segregation pattern, T1 generation had WT,...
09 September 2019 9,005 3 View
One of my rice mutant produce more than 50% seeds as empty seeds. The seeds when opened look like in the attached picture. Can anyone explain the phenotype in the attached picture. I am not sure...
05 May 2019 4,705 5 View
I want to select genes that are most affected (differentially expressed) in a mutant. I have the RNA seq data for the differentially upregulated and downregulated genes in an already published...
10 October 2018 7,450 10 View
I want to check the role of a gene in rice fertility. One experiment is to count total pollen number per anther. But I am not successful in collecting the total pollens. Please suggest a possible...
08 August 2018 8,055 5 View
Hello everyone, Using CRISPR CAS9, I got homozygous +1 mutants for a rice gene. The CAS9 target lies in the middle of the gene. After mutation there is no stop codon in gene body. Only one in UTR...
05 May 2018 7,668 5 View
The problem is that I am trying to clone 20bp sgRNA target sequence for CRISPR CAS9 into an Agrobacterium binary vector pHun4c12 which has a size of 13.5 kb which is large enough. The vector has...
10 October 2017 389 9 View
