25 Questions 51 Answers 0 Followers
Questions related from Azeem Mehmood Butt
Dear all Could anyone please tell me how to integrate epidemiological data with region? I am looking for a way/tool to show epidemiology of a disease and its features on a country map? For...
09 September 2016 8,504 7 View
Hello everyone I would like to ask question about situations where gene expression values are used to correlate with clinical features of samples under investigation. For example, in an...
01 January 2015 4,039 5 View
I am using the QIAGEN miRNeasy kit for total RNA isolation from tissue and serum samples as per manual instructions. However, I am facing issues with the phenol contamination and the 260/230...
05 May 2014 279 7 View
I would like to know if there is anyone who has worked specifically with QIAGEN cel-miR 39 spike-in using the QIAGEN miRneasy mini kit.
05 May 2014 9,456 3 View
In codon usage studies, it is observed that a correlation between ENC and GC3 values is plotted as a curve following Wright's, 1990 method and hypothesis. However, what should be the case when a...
12 December 2013 755 1 View
I would like to have your opinions on rights of students when they are enrolled in different degree programs considering following case as an example: 1. A student is studying for his/her higher...
09 September 2013 9,268 9 View
I am trying to perform the correspondence analysis between the set of values of GC3 and ENC, given in the attached Microsoft Excel sheet. The problem is that I have applied trend line (polynomial)...
08 August 2013 6,105 2 View
There are several target prediction tools but they are limited in terms of options that we can select humans, mouse etc genomes. What if the genome sequence of any other organism, which is not...
07 July 2013 9,947 2 View
Hello everyone Could anyone please tell me is it possible to isolate COX protein from serum or whole blood samples for western blotting?
03 March 2013 8,431 2 View
Please suggest a reliable free software/server for annotation of newly sequenced viral genomes
02 February 2013 2,290 7 View
Please tell me any tools/servers available which can be used to draw figures for graphical representation of annotated genomes (bacterial and viral).
02 February 2013 9,748 8 View
I would like to know how to download GFF or GTF files of annotated full length viral genomes from NCBI?
02 February 2013 3,239 4 View
I am trying to do phylogenetic analysis of upto 100 full length viral genomes (each consisting of 34000 - 36000 bp). However, I am unable to perform alignment and generate trees by using MEGA5...
01 January 2013 5,160 22 View
I have cloned a transgene and promoter in plant expression vector. Cloning of transgene was confirmed by PCR and restriction digestion and its orientation has also been confirmed. Promoter was...
12 December 2012 467 8 View
I have to isolate total RNA from needle biopsy tissue samples for the purpose of microRNA profiling by using next generation sequencing. I have purchased Ambion mirvana kits but after repeated...
12 December 2012 8,941 11 View
Is there any software(s) which allows to import data from different real time PCR machines for the purpose of comparison and quantification?
11 November 2012 9,344 3 View
In biorad IQ5, calibration and dye settings are stored in calibration files. So is it possible to use multiple calibration files when different labs use different PCR plates and sealing tapes/caps.
11 November 2012 5,927 1 View
I have to perform expression profiling of microRNAs from serum samples. As there is no universal normalization method yet available, I intend to use synthetic c.elgans miR-39 microRNA for...
11 November 2012 9,901 8 View
I have to design primers based on stem-loop primer method for different miRNAs as I am interested to check their expression in my clinical samples. Can anybody please tell me which miRNA sequence...
08 August 2012 3,207 14 View
Does anybody know of a database which specifically contains 16s rRNA sequences from different microbes?
03 March 2012 9,310 4 View
Is there any group or personals working on hepatitis C and microrna? Our group is interested in running a collaboration project. Those who are interested please send me email at : [email protected]
02 February 2012 3,775 1 View
Hi all, Can anybody please tell me what is the difference between RNA-seq and transcriptome sequencing? I want to perform parallel expression profile miRNA and mRNA from same biological samples. I...
01 January 2012 8,332 15 View
Hi all, Could anyone please tell me how to find/predict whether a specific bacterial gene is present on leading stand or lagging strand?
01 January 2012 682 1 View
Dear All, I would like to know what is most appropriate protocol for storage of intestine biopsies in Liquid Nitrogen? 1. Take sample. 2. Put it in per-chilled cryovial. 3. Immediately place...
01 January 2012 5,465 11 View
01 January 2012 2,118 5 View
