Can anyone suggest me the DAPI concentration (less than 300nM) that works good with IHC?

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Leya Mwense

Hello, i suggest testing a small range of concentrations (e.g., 50, 100, 200 nM) to optimize for your specific samples. Also, please do invest in VECTASHIELD® Antifade Mounting Medium with DAPI for fluorescence. You will only need 25 µl on you slide and doesn't usually get overstain incase you dont get your desired stain.

Jeffrey P Cheng

VECTASHIELD® Antifade Mounting Medium with DAPI is excellent, highly recommend. Otherwise serial dilutions are the way to optimize.