Asim Zahoor Abbasi

4 Questions 6 Answers 0 Followers

Questions related from Asim Zahoor Abbasi

What could the reason for failure of EdU-labeled DNA to click with biotin-PEG3-azide and enrich by streptavidin?

I have labeled my replicating bacterial DNA with 5-Ethynyl-2'-deoxyuridine (EdU) and want to click it with biotin-PEG-3-azide followed by enrichment with magnetic streptavidin beads. So far, I...

07 February 2024 5,417 0 View

Analyses of Plasmid sequence?

We want to analyse whole genome of Bacteria including Plasmid. I have extracted DNA with DNA extraction kit. I want to know whether we have to also add plasmid obtained by miniprep only extracted...

05 December 2019 7,546 2 View

Can any one provide me optimized Multiplex PCR conditions for detection of Extra-Intestinal Pathogenic E. coli?

Hi, I am working on Bovine mastitis and identification of ExPEC genes is one of my objectives in research. Could any body please provide optimised Multiplex PCR conditions (primer sequences and...

23 August 2019 2,137 1 View

How to confirm if Chromatogram peaks of your 16s rRNA gene sequence are good enough for analysis?

Hi, I am doubtful about quality of 16s rRNA reverse sequence. I want to know how to confirm sequence quality. Please find attached file below to ascertain whether it is good enough or not.

11 February 2019 532 4 View