I have to covalently conjugate thiolated ss-oligo probes (10-20 nucleotides long) on to the surface of amino-functionalised magnetic beads. I have been looking up papers on that and I came across many cross linkers which have been used for this reaction like N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP), 3‐maleimidopropionic acid N‐hydroxysuccinimide , sulfosuccinimidyl 4-Nmaleimidomethyl cyclohexane-1-carboxylate and Succinimidyl 4-[malemidophenyl]butyrate. Could someone please suggest which of these would be the best suited or direct me to a study (if any) which compares the use of different cross linkers on the reaction efficiency?
Thanks!
Arti Tyagi
Stjepan Krešimir Kračun
I had another question if you could please help me out? The paper which used sulfosuccinimidyl 4-Nmaleimidomethyl cyclohexane-1-carboxylate (SMCC) as a cross-linker also uses sulfo-NHS acetate later on to block the unreacted sulfo-SMCC. But this sulfo-NHS acetate is quite expensive and I cannot get it now. So what do you suggest, should I look for alternate blocking reagents or instead use one of the other cross-linkers?
1 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Column
More Arti Tyagi's questions See All
Similar questions and discussions