Arif Mahmood

10 Questions 20 Answers 0 Followers

Questions related from Arif Mahmood

Why TFEB is translocated to Nucleus in control cells?

Hi, I am co-transfecting Hela cells with TFEB-GFP plasmid and Plasmid of Interest. However. In my control cells the TFEB also mostly translocate to nucleus. Although, I replaced medium 1h before...

10 September 2021 5,916 0 View

Why the total histone H3 or H4 expression is high or low in western blot?

Hello, I am treating SH-SY5Y cells with different drugs of which cause Parkinson. during western blot i see that acetylation of H3 or H4 increase decrease which is fine but why my total level of...

12 August 2019 423 2 View

Why my negative control have high expression proteins?

I am using DMSO treated SH-SY5Y cells for negative control but i get high expression of protein in it. I treated my cells with different toxic drugs to induce Histone modifications but. My...

17 October 2018 7,329 1 View

Can i add two or more antibodies to one sample for WB?

I am doing experiment on SH-sy5y cells treated with drugs but i am using different antibodies of chromatin. my question is whether i can add these Ab to one sample or i have to do one by one with...

18 August 2018 3,172 3 View

Designing of Microbes to rescue gene Phenotypes?

Hi All, I want to to know whether can i design such microbes to rescue gene phenotypes (The gene which mutations leads to human diseases) Your guidance will be highly appreciated.

23 May 2018 6,298 4 View

Touchdown PCR for primers?

Hi, I am using both pairs gRNAs as a insertion part in the vector. So i have to do touchdown pcr for these pairs to annealed them... so what you will suggest for touchdown pcr steps and cycle...

08 November 2016 3,957 3 View

Choosing Esp31 Restriction enzyme?

Hi, I am carrying molecular cloning using classical methods. I have check enzymes which provide an understandable pattern so any one tell me if i list features of Esp31 enzyme so what will be the...

03 November 2016 7,979 3 View

What is the Criteria for selection of potential SNPs for study?

Hi, Can any one guide me that what is the criteria for selection of potential SNPs in receptors to further characterize it. Thanks

30 September 2016 1,747 7 View

How to differentiate between Heredity Spastic Paraplegia and Cerebral Palsy?

I am working on a family with autosomal recessive in which affected members have CP characters so how will i differentiate between whether it is Cerebral Palsy or Heredity Spastic Paraplegia?

01 February 2016 2,045 8 View

Any one can help me in identification of this syndrome.?

Can any body help me in identification of syndrome. Two female patients are affected phenotypes are Seizure, one patient have 70% deafness, cant walk, cant speak, mental retardation, low calcium...

14 October 2015 5,774 6 View