Anuj Kumar Singh

5 Questions 9 Answers 0 Followers

Questions related from Anuj Kumar Singh

Why only qPCR data is not sufficient for a study on caner cell related genes ?

I am doing research on cancer and its probable association with few genes which I am showing with qPCR data and it is not possible for me to do WB for that and if I study any pathway. Is it...

20 January 2017 8,128 1 View

Why CRISPR/Cas9 system is not able to knockout my gene of interest ?

I want to knockout a gene using CRISPR/Cas9 system but so far unable to do it. I am applying this on human cancer cell line and 10% FBS DMEM with antibiotic media, Lipofectamine transfection...

14 January 2016 1,978 10 View

Can I propagate CRISPR plasmid by transforming E.coli ?

I am going to use CRISPR/Cas9 system for knockout a gene. CRISPR is in the form of a plasmid for an idividual gene. My question is that can I propagate CRISPR plasmid by transforming E.coli ? If...

05 October 2015 3,707 8 View

Where can I get an oral cancer cell line?

Hi I am working on oral cancer and I want get cell line other than SAS. Is there any one in India can provide me other cell line to help my study? I will be very thankful to the person.

14 April 2015 589 2 View

Can someone help me with an oral cancer (SAS) cell culture problem?

I am facing a problem in cell culture- Recently our lab shifted to powder based media (DMEM) from liquid media. I am trying to grow an oral cancer cell line (SAS) but I found that my cells are...

04 February 2015 6,051 10 View