Anuj Kumar Singh

10 Questions 10 Answers 0 Followers

Questions related from Anuj Kumar Singh

Why I see fluctuation of cerebral artery pressure myography traces?

I am working on cerebral arteries (middle and posterior mainly), isolated from mice for pressure myography experiments where I observed fluctuation of cerebral artery often. I am using PSS with...

27 April 2023 356 0 View

Ca2+ influx and cancer: Which type of graph is good to present the influx ?

I want to present my work of Ca2+ influx which I am doing with emptying of store by using thapsigargin(Tg) and then providing 3mM CaCl2 from outside after loading with Fura-2AM in a cancer cell. I...

01 April 2017 316 1 View

Why only qPCR data is not sufficient for a study on caner cell related genes ?

I am doing research on cancer and its probable association with few genes which I am showing with qPCR data and it is not possible for me to do WB for that and if I study any pathway. Is it...

20 January 2017 8,196 1 View

Can I see my GFP positive mammalian cell in Gel doc system ?

My mammalian cells are able to give GFP as reporter signal but now my fluorescent microscope is not functional. In this case can I see my cells in gel doc system ? If I do this is it possible to...

08 July 2016 3,879 1 View

Rescue experiments are crusial when we do silecing experiment by using RNAi or shRNA or CRISPR-Cas but is it possible through a drug only ?

I am going to do an experiment where I want to check whether removal of the drug is able to restore the cellular function or not. I am searching literature but so far I did not get it if any. Can...

27 June 2016 3,555 1 View

Why CRISPR/Cas9 system is not able to knockout my gene of interest ?

I want to knockout a gene using CRISPR/Cas9 system but so far unable to do it. I am applying this on human cancer cell line and 10% FBS DMEM with antibiotic media, Lipofectamine transfection...

14 January 2016 2,023 10 View

What will happen if I use antibiotic in my CRISPR transfection and cell culture media ?

I want to transfect CRISPR/Cas9 plasmid in a human cell line to knockdown a gene and as per the protocol I should not use antibiotic at any stage but I am getting bacterial contamination at some...

05 December 2015 6,468 6 View

Can I propagate CRISPR plasmid by transforming E.coli ?

I am going to use CRISPR/Cas9 system for knockout a gene. CRISPR is in the form of a plasmid for an idividual gene. My question is that can I propagate CRISPR plasmid by transforming E.coli ? If...

05 October 2015 3,738 8 View

Where can I get an oral cancer cell line?

Hi I am working on oral cancer and I want get cell line other than SAS. Is there any one in India can provide me other cell line to help my study? I will be very thankful to the person.

14 April 2015 623 2 View

Can someone help me with an oral cancer (SAS) cell culture problem?

I am facing a problem in cell culture- Recently our lab shifted to powder based media (DMEM) from liquid media. I am trying to grow an oral cancer cell line (SAS) but I found that my cells are...

04 February 2015 6,100 10 View