12 Questions 13 Answers 0 Followers
Questions related from Ankur B. Sharma
I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...
24 February 2021 9,483 1 View
Hi Everyone, I have used Phalloidin to label F-actin in ethanol fixed cells ( I know formaldehyde might have been a better choice for fixation). I am new to new to this and I am experiencing that...
22 November 2017 8,929 1 View
Hey Everyone, I am trying to sort hepatocytes after collagenase based perfusion of mouse liver. I am using RBC lysis buffer to remove RBCs from my sample and antibody based gating out of WBCs....
17 November 2017 9,553 5 View
Hello All, I just started using HepG2 cell lines and I would like to know the culturing conditions. I have read through the ATCC guidelines for this cell line and they recommend EMEM+10% FBS....
03 October 2017 1,213 4 View
Hi everyone, I am working on C57BL/6 mouse and I was wondering if anyone of you knows what is the average size (or size distribution) of hepatocytes of these mouses? Any link to a published report...
11 September 2017 3,848 7 View
Hi, I am planning to sort animal cells (after perfusion) and fix them. Generally, I am collecting sorted cells in a buffer, centrifuge them and fix them using 70% ice-cold ethanol. I was wondering...
27 June 2017 9,198 2 View
Hi guys, I am using Hoechst 33342 for sorting live cells and using reserpine to improve Hoechst staining (I have observed it improves the staining and there is a logical explanation to that). My...
08 June 2017 4,333 1 View
will medium containing 10% FBS inactivate DNase 1?
15 October 2016 4,062 0 View
I am interested to test the efficacy of my compound on pancreatic cancer cell lines. NCCS pune dont have them. Can someone share their freeze or help me to get one. ( except ATCC Obviously )
27 May 2016 3,005 2 View
I am trying to purify a polar compound ( polarity analysed based on Rf value in DCM system ) ethyl acetate cell free extract of a marine fungus and before running a column , i want to figure out...
25 May 2016 9,722 2 View
recently i came across an article using deletion-specific targeting (DST) vector for cancer therapeutics . I just wanted to know the principle and detailed mechanism of deletion-specific targeting...
09 February 2016 5,789 0 View
Hey guys, I wanted to sort Binucleated and tetraploid cells (from tissue or cell lines )using some techniques like FACS (rather than microscopic techniques ). Can you guys suggest...
01 January 1970 3,303 15 View
