Andrew You

5 Questions 4 Answers 0 Followers

Questions related from Andrew You

Best way to acclimate virus injected ChAT-cre mouse to modified T-maze?

So I had no problem training uninjected C57BL/6J mouse, but I am currently trying to work with injected (brain) ChAT-Cre mouse and having difficulties. I'm using the modified T maze (figure 8)...

24 June 2019 6,928 1 View

Best way to acclimate virus injected ChAT-cre mouse to modified T-maze?

So I had no problem training uninjected C57BL/6J mouse, but I am currently trying to work with injected (brain) ChAT-Cre mouse and having difficulties. I'm using the modified T maze (figure 8)...

06 June 2019 2,579 1 View

Why do I see double peak melting curves for het mutants in qPCR?

The het cDNA is a result of CRISPR/Cas9 knock ins/knock outs (F0) crossed with WT to produce F1s. I analyse the DNA of the F1s and notice that all the het's have two peaks while WT and later -/-...

11 November 2017 2,919 0 View

What can I do to refine my fragments for Gibson Assembly?

I'm currently trying to assemble a plasmid with my insert. The plasmid is the SAGFF (6293bp) and my inserts I want to insert (individually) are 2300bp and 3000bp. I have used both a 1:2 and 1:3...

12 September 2017 7,737 5 View

What can I do to refine my fragments for Gibson Assembly?

I'm currently trying to assemble a plasmid with my insert. The plasmid is the SAGFF (6293bp) and my inserts I want to insert (individually) are 2300bp and 3000bp. I have used both a 1:2 and 1:3...

09 September 2017 6,805 6 View