Andres Fernandez Gil

6 Questions 8 Answers 0 Followers

Questions related from Andres Fernandez Gil

Anyone has experience using antibody labelling kits?

We are trying to purify and analize by FC cells with a cell surface marker, but the only antibody with reactivity against this in mouse is not conjugated. Anyone has used antibody labelling kits?...

11 November 2017 1,585 2 View

Does anyone find problems anesthetizing NSG mice with ketamine/Xilacyne?

we are performing intracraneal injection of in NOD SCID Gamma mice, and we are finding unusual problems with anesthesia, loosing 75% of mice during surgery or next following days. From last 8...

05 May 2017 663 1 View

Nestin GFP mice genotyping?

Hi, I have to genotype nestin-GFP mice. But I've found that there is only available a PCR reaction that only can tells if the construction is present in the mouse, but can not distinguish between...

01 January 2017 7,935 2 View

Does anyone know the best puromycin concentration to select gliobastoma cells transformed with lentivirus?

I have infected two glioblastome cell lines: GL261 and U373, with a lentivirus that express resistance to puromycin. Anyone knows the best dosis and time of treatment? I've found some data using...

04 April 2015 8,206 11 View

Does anyone have any feedback from using the Thermo GeneJET Maxiprep kit?

I am considering using Thermo GeneJET Maxiprep kit, it´s an obvious question of price. Anyone has use this before? and specially using the plasmid preparation to produce lentiviruses? Thanks a lot!

04 April 2015 5,110 4 View

Can the specificity of Lentiviruses change between different stocks?

I am producing several lentiviruses for different purposes, but I am finding some amazing results: an old stock of certain virus can infect with no problem mouse and hume glioblastome cells (GL261...

12 December 2014 1,653 2 View