Anar Alshanbayeva

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Questions related from Anar Alshanbayeva

Does anyone know a good Argonaute2 antibody to work on mouse plasma samples, preferably compatible both with IP & Western?

I currently have rabbit primary AGO2 antibody; however, it does not give strong bands in mouse plasma samples and this antibody has never been tested on mouse samples before. I see AGO2 band,...

04 April 2019 8,996 4 View

Best method (affordable for many samples) for cDNA synthesis from low input (0.1pg-0.5pg-1ng) purified small RNAs?

Does anyone know of a good cDNA synthesis method for small RNAs of low input (0.1pg-0.5pg-1ng) for routine usage? Thank you

04 April 2019 9,741 1 View

Are assessment of hemolysis by absorbance at A414nm or calculations by M.Harboe method necessary?

Even mouse plasma that has (by observing visually) absorbance higher than 0.3 at A414nm. According to publications, this is a high level of hemolysis in humans. Does the same cutting point (0.3)...

07 July 2018 5,863 0 View

Is there a way to take into account the effect of hemolysis level to mouse plasma total RNA sequencing?

Several publications indicate hemolysis levels alter differential expression of a number of miRNAs in human plasma, in some cases up to 40-88% of miRNAs (does anyone have reference to such mouse...

07 July 2018 6,116 0 View