I work with Drosophila samples. I tagged my protein of interest with mKate2 tag and want to immuno-stain against this tag. The problem is that I also have TdTomato expressed in my samples. I would really appreciate if anyone here can recommend me a commercial antibody that would efficiently recognize mKate2 but not TdTomato or RFP.
https://evrogen.com/products/mKate2/mKate2.shtml
Hello Alina, above you will find a link where you can get alot of informations even about a maxbe suitable antibody. If your labeling is strong enough you can see in in the microscope without immunohistochemical intensification. You need therfore a Cy5/TexasRed/ alexa 647 Filtersystem. Good luck!
Hello Ute Neubacher
The company is situated in Russia, and, unfortunately, due to the war in Ukraine their products are not supplied to my country anymore. I have to use immunostaining because I am currently performing expansion microscopy, and the fluorescent protein tags don't survive the protocol very well.
