6 Questions 9 Answers 0 Followers
Questions related from Alexandre Dias
Hello everyone! I've recently ordered a DuoSet™ ELISA kit from R&D Systems, and while reviewing the protocol, I came across a few points that raised some questions. If anyone has experience...
22 April 2025 3,609 2 View
Hello everyone, After conducting a proteomics experiment, I identified some targets I want to validate. So, I embarked on a challenging journey to find reliable ELISA kits—and it turned out to be...
13 February 2025 8,926 7 View
Hello, I will need to separate proteins from the nucleic acids' fraction from extracellular medium samples (which have low concentrations of both). Does anyone know if I could still use the...
26 July 2022 8,958 0 View
Hello. Do anyone know how to dissociate mice stomachs in a single cell suspension? I tried with trypLE but I could not form a pellet. When using DNAse which are the optimal concentrations. If...
29 January 2018 1,022 0 View
I'm doing a gastric organoids culture, separating the corpus from the antrum. I plated the glands, and 3/4 days after plating them, and having an apparently normal development into organoids, they...
27 February 2017 7,640 13 View
01 January 1970 5,636 0 View