Alessandro Strofaldi

5 Questions 6 Answers 0 Followers

Questions related from Alessandro Strofaldi

DNA purification from ATCC 25922 E.Coli strain with QIAGEN kits?

Hi everyone, We've got a plasmid we want to purify from E.Coli strain ATCC 25922. We usually use QIAGEN plasmid purification kits but we've never purified DNA from this particular strain. Would...

21 May 2024 3,765 1 View

Can nitrosamines be formed by oxidation of secondary amines using hydrogen peroxide?

Hi! I'm working on a secondary amine with one R group containing nitrogen (R-NH-R'; R'=NCH2). I need to know if it's possible to form nitrosamines by oxidation of the NH group by hydrogen peroxide...

28 September 2022 7,264 0 View

PDB Deposition: Resolution is lower than overall refined resolution?

Hi All, I’m preparing a structure for deposition on the PDB; everything is ready but I got a red alert in the Refinement statistic page. The error is relative to refinement of Shell 8 and it says...

09 July 2021 8,694 2 View

Positive or Negative Spherulites?

Hi, I'm trying to determine if my protein spherulites are positive or negative by polarized light microscopy. This involves the use of a 530 or 550nm retardation plate (RP) inserted before the...

13 January 2021 6,171 0 View

AKTA purifier error: No Uninet connection between controller and the system?

Hey there I'm experiencing a similar problem hope someone can help: Suddendly the Uninet controller stopped connect to the system leading to continuously Uninet error 2000 coming out (Uninet...

20 April 2018 4,826 3 View