Alessandro Strofaldi

7 Questions 8 Answers 0 Followers

Questions related from Alessandro Strofaldi

SDS-PAGE problem: horizontal line during run preventing from appropriate band resolution?

Hi everyone, I'm having a similar issue so I'll use this topic (even if I think the problem may be totally different). When running gels, I'm often having a very narrow horizontal line which often...

18 June 2024 286 1 View

DNA purification from ATCC 25922 E.Coli strain with QIAGEN kits?

Hi everyone, We've got a plasmid we want to purify from E.Coli strain ATCC 25922. We usually use QIAGEN plasmid purification kits but we've never purified DNA from this particular strain. Would...

21 May 2024 3,847 1 View

Can nitrosamines be formed by oxidation of secondary amines using hydrogen peroxide?

Hi! I'm working on a secondary amine with one R group containing nitrogen (R-NH-R'; R'=NCH2). I need to know if it's possible to form nitrosamines by oxidation of the NH group by hydrogen peroxide...

28 September 2022 7,297 0 View

PDB Deposition: Resolution is lower than overall refined resolution?

Hi All, I’m preparing a structure for deposition on the PDB; everything is ready but I got a red alert in the Refinement statistic page. The error is relative to refinement of Shell 8 and it says...

09 July 2021 8,734 2 View

Positive or Negative Spherulites?

Hi, I'm trying to determine if my protein spherulites are positive or negative by polarized light microscopy. This involves the use of a 530 or 550nm retardation plate (RP) inserted before the...

13 January 2021 6,205 0 View

AKTA purifier error: No Uninet connection between controller and the system?

Hey there I'm experiencing a similar problem hope someone can help: Suddendly the Uninet controller stopped connect to the system leading to continuously Uninet error 2000 coming out (Uninet...

20 April 2018 4,865 3 View

30kDa endogenous contaminant in E.Coli colture?

Hey everyone, I'm trying to obtain an enough pure batch of the protein I will work on but I can't get rid of a 30kDa contaminant it's seem to be produced endogenously by my cells. Briefly the...

12 March 2018 3,096 4 View