12 Questions 16 Answers 0 Followers
Questions related from Aleksandar Dishkelov
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
25 July 2024 9,310 3 View
Hello, During skeletal muscle contraction and during the actin–myosin ATPase cycle there is release of Pi and ADP. Can anyone tell me and ideally suggest a reading list of where they actually...
15 November 2023 6,560 4 View
Hello, I have no prior experience with growing cell line nor differentiation toward specific cell type. Currently I have C2C12 cell line which I need to grow and then differentiate it towards...
03 November 2023 4,177 9 View
Hi all, bit confused so I need a bit of explanation. How do I dilute a solution, lest say I have a working solution of 200 uM, and I wish to make it to lets say 20 uM. 1M =1000mM=1000000uM...
04 December 2022 5,626 0 View
Hello, I am interested if I can observe microclots using whole blood (rather than PPP) under fluorescence microscope? To increase the lifespan of the samples I can fix the whole blood via...
23 April 2022 499 2 View
Hello, I'm little confused about the secretion of IL4. In some papers it is mentioned that B cells secrete IL4, on other that it is Th cells, or even basophils. Can you also provide me with...
14 August 2018 9,457 3 View
Hello everyone. I have begun on analyzing platelets using a flow cytometry. As I have never done analysis of this cell type, can you please help me out. For now I am using markers CD61...
01 January 1970 8,453 1 View
Hello everyone, I am currently using BD LSRFortessa™ X-20 Cell Analyzer, and analysing the activation of platelets using whole blood. Enough analysis has been done, however, I need help with...
01 January 1970 3,668 7 View
Hello, I am investigating the activation of platelets with the use of flow cytometry. I am specifically looking at their activation when agonists such as ADP, collagen, and thrombin are introduced...
01 January 1970 1,068 7 View
Hello, I am interested in the alternative options for blood anticoagulant. Heparin, EDTA, sodium citrate, all of them affect one way or another the coagulation. I am testing platelet function...
01 January 1970 1,230 0 View
Hello, I am interested if anyone has investigated platelet using the fluorescence microscope EVOS. Would it be possible to use whole blood samples, where platelets are the main focus, and see...
01 January 1970 811 1 View
Hi all, can anybody confirm that my calculation is correct? 250 pg/ml to micromolar ( MW is 183.20) 250pg/ml x (1mg/1000 micrograms)x(1mol/183.20g)= 1.362x10^-12 mol/microliter mol/microliter...
01 January 1970 6,961 2 View
