Ahmad Rizqi Kurniawan

6 Questions 5 Answers 0 Followers

Questions related from Ahmad Rizqi Kurniawan

Should I re-spot the white colonies in new blue-white screening plate after first screening?

My supervisor suggested me that I should re-spot the white colonies in new plate after performing first blue-white screening. This step allows white colonies to develop the color of colonies...

08 August 2015 7,879 3 View

What is the role of scaffold-acting domain in protein?

I still do not quite understand a domain in protein that acts as a scaffold. What is simply the main role of this domain? What will happen to this domain or protein if the corresponding gene is...

26 June 2015 6,196 2 View

What is the function of helical domain of PI3Kα?

I do not quite understand about the function of helical domain of PI3Kα. The following paper stated that this domain acts as a scaffold for other domains of p110. I do not have an experience about...

18 June 2015 6,253 1 View

Why does the color of my transformant colonies turn into blue?

I already have performed the blue white screening and got the white colonies (transformants). I cultured the transformants onto blue white screening plate again (already containing X-gal,...

04 June 2015 7,370 3 View

What antibiotics should I use and why?

I am using Escherichia coli DH5alpha as competent cells to insert recombinant plasmid. I use pGEM-T Easy Vector from Promega. To ensure my competent cells are not contaminated, I (as my supervisor...

19 May 2015 7,149 12 View

Which agarose percent should I use to quality check genomic DNA by gel electrophoresis?

My supervisor usually use 1% agarose gel to check the DNA genomic quality of human cancer cells. But in this attached book, it is stated that it should use agarose gel with percentage less than...

19 March 2015 2,442 11 View