16 Questions 68 Answers 0 Followers
Questions related from Adam Hughes
Following this guide, I get the impression that EDC/NHS activation is used to link to primary amines to carboxyls. Wouldn't it also activate secondary amines on arginine and other amino acids? If...
20 May 2015 9,319 7 View
Imagine a paper X builds a new sensor, and then uses the new sensor to detect protein Z. When citing paper X, I might want to point out that they've build a new sensor. EG:"New sensors have been...
09 March 2015 6,149 45 View
In spectroscopy, we generally look at an object of some composite color (ie a white light, a red cuvette of nanoparticles, a green lawn) and separate the composite colors into a spectrum. For...
19 February 2015 726 3 View
I've done calculations using Fresnel's equations and the transfer method matrix calculation on layers of thin films. These ray-optics calculations make sense to me, but I don't have a good...
27 January 2015 9,545 2 View
I'm modeling a system which requires effective mixing theories in two places. First, I'm modeling a shell of proteins around gold nanoparticles as a homogenous mixed material of protein (n=1.5 or...
22 December 2014 4,602 3 View
Hi, I'm trying to perform an honest assessment of the state of open-source software for simulations of plasmonic systems. For example, simulating the transmittance/reflectance off of an optical...
21 December 2014 9,678 5 View
Hi, We're doing some LSPR studies measuring antigen-IgG binding, and I'm trying to learn the general properties of IgG's. From the few I've studied carefully, they have similar masses...
07 November 2014 174 4 View
Hi, We've been using EDC/NHS activation to covalently attach proteins to a carboxylated gold surface with fair success. We'd like to begin immobilizing IgG's, and I'm worried about controlling...
09 September 2014 3,348 11 View
We are looking at set of protein sequences from the purple sea urchin, and trying to identify the function of a subset of 200 or so proteins in the genome that have a very specific domain. From...
22 May 2014 373 19 View
We are looking to measure a specific interaction between NeutrAvidin and an antibody. We've read that Streptavidin does not appreciably bind antibodies specific to Avidin, but there is no...
16 May 2014 3,165 3 View
I have several stock powders that I'd like to store for as long as possible in a refridgerator. We have limited access to N2, so what I've been doing is distributing the stock powder into smaller...
17 April 2014 1,559 1 View
We've developed a biosensor based on plasmonic resonance of nanoparticles and would like to perform antibody-antigen conjugation experiments. With almost no formal biology experience, it's really...
17 April 2014 1,196 7 View
I use pirhana to clean glass fibers and slides for gold nanoparticle self-assembly experiments. The piranha frees hydroxyls on the glass, which then allow for covalent binding to various chemical...
19 October 2013 8,270 27 View
I am interested in using optical fibers with sharpened tips for detection in biochemical systems. We are coating the tips with metallic films or nanoparticles. I've noticed that groups that make...
10 May 2013 5,771 2 View
This is one of those gritty details that never makes it into a paper. I often use Amine-capped silanes (such as 3aminopropyltrimethoxysilane- APTMS) in hopes of constructing monolayers of gold...
29 January 2013 1,111 4 View
I have been successfully depositing home-made, 30nm citrate-capped gold nanoparticles onto amine-functionalized glass surfaces. Recently, I noticed that a couple other batches of particles (one...
27 January 2013 9,705 7 View
