I am trying to get SEM result for Yersinia enterocolitica from SWCNTs based biosensor.However I am not getting expected result.
Procedure was..
1) CNT immobilization
2)Linker application
3)Antibody immobilization for overnight
4)Yersinia immobilization
5)Finally observe.But not clear image.....
Rostislav Váňa
What do you mean by not clear image, please provide the image to see the issue you are facing and what do you want to see.
And please write the conditions you are using (type of the microscope, HV, beam intensity,..)
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António Maximiano Fernandes
By SEM is not difficult to image bacteria. Maybe the antibody concentration is so high, or has effect on Yersinia membrane. Try to decrease the concentration of antibody
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