8 Questions 41 Answers 0 Followers
Questions related from Abhijit Babaji Shinde
I am trying to stably express either a WT or a mutant form of my protein of interest under the control of doxycycline (using pTRIPZ vector) in C2C12 and ST2 cells to study it's role in bone...
05 May 2019 1,207 2 View
I have some mutant mice which show elevated blood glucose levels as compared to controls. So I just wanted to take valuable opinions of all of you about which factors relating mainly to beta-cells...
08 August 2016 3,336 6 View
I need to extract RNA from islets of Bl6 mice. So I was just wondering which is the best kit for extracting RNA from a low number of cells (around 100 islets only)? If anybody has experience with...
11 November 2014 8,324 6 View
I came across two procedures wherein the first collagen digestion steps are same but one uses handpicking for purification while the other uses ficoll gradient. I was just wondering whether...
03 March 2014 8,814 21 View
These days we are facing lots of contamination problems in our cell cultures and we have been trying and have already tried lot of things to get rid of it. Just wanted to ask you people about the...
03 March 2014 3,561 31 View
I want to measure the mitochondrial ROS status in the primary hepatocytes of WT vs KO mice that I have. I have already tried some ro-GFPs, but transfection is the problem there. I have also tried...
02 February 2014 7,641 20 View
Currently I am investigating the effects of knocking down Pex-5 from hepatocytes using primary hepatocytes from WT and liver specific Pex-5 KO mice livers. As these primary hepatocytes are...
08 August 2013 4,272 15 View
I am interested in looking at the ROS in the cytoplasm and mitochondria using some ro-GFP probes for which I have to transfect primary hepatocytes with these plasmids. I have already tried...
08 August 2013 10,047 10 View
