01 January 1970 4 4K Report

How do you want your students to remember you?

More Colleen Maykut's questions See All
What percent HCl should an acid bath have in order to clean glassware to remove phosphorus?

There is a USEPA standard on this I believe, but I am having trouble finding it. Our lab washes dishes and we place dried dishes in the HCl acid bath to remove contamination but focus especially...

09 August 2023 2,031 4 View

Should a material with a lower Tg have a lower Stiffness and modulus than one with higher Tg?

Tg vs stiffness and elastic modulus

20 July 2023 9,382 1 View

Can you help me suggest a theory or model on information literacy?

I am working on a research about developing an information literacy program module for high school students.

25 September 2021 6,962 6 View

What conventions are followed for finding particle sizes settled via ultracentrifugation when multiple materials (soil/organic matter) are targeted?

I am using a Beckman ultracentrifuge to settle out colloids (less than 1um in diameter, with filtration occurring beforehand) from soil column leachate and trying to figure out the lower limit of...

12 May 2021 1,260 0 View

How can I take subsamples of water from a larger sample in a way that allows me to measure the iron content of the subsample?

I learned how to take subsamples with a magnetic stir bar, but I noticed iron particles stick to the bar, and I am having trouble finding a standard method to solve that problem. How can I take a...

04 February 2021 5,152 1 View

What are some methods to measure nutrient contents in manure using ICP-OES?

I am planning to analyze manure samples for Total Kjeldahl NItrogen, Total Kjeldahl Phosphorus, and dissolved reactive phosphorus using and AQ2 analyzer. I would like to also look at...

27 August 2020 7,056 4 View

Can anyone recommend a good ICC antibody specific for human TMEM119?

Mouse antibodies on the market appear to be better than the human antibodies, which seem to label poorly. Any experience with antiTMEM119 antibodies for labeling human microglia would be helpful....

22 July 2020 7,380 1 View

Can anyone recommend a microglia-specific promoter to drive gene expression in steady and activated state, for designing OE AAV vectors (not TMEM119)?

Looking for a good microglia-specific promoter, other than TMEM119, for the purpose of plasmid design, to be packaged in AAVs. In vitro and in vivo applications.

22 July 2020 7,403 0 View

Is there a microglia specific marker to differentiate from macrophages (that is not TMEM119)?

Hello - I'm working with stem cell derived microglia. When adding to 3D cultures I'm not getting a signal for TMEM119, which based on literature could be due to the immaturity of the cells (where...

19 February 2020 1,657 3 View

Synthetic Materials with Acoustic Impedances of 1.53 and 1.69?

Hello, My name is Colleen O'Brien, a senior biomedical engineering student.For my senior project I am required to build a model of human abdomen to validate a device we are developing. I still am...

04 December 2019 3,467 3 View

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Could anyone recommend software for drawing the physical structure of nanoparticles?

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