I am trying to stimulate T47D and SKBR3 cells with 10ng/mL EGF for my wound assay. Not many people that I know of in my lab have stimulated cell lines other than BT20s with EGF, but it works at 10ng/mL after 12 hours. I am not getting good stimulation of migration with EGF for T47D and SKBR3 cells. Are there other growth factors that are more commonly used with these cell lines, like heregulin or TGFa?

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