We have always frozen our HIV stock with ethanol/dry ice bath, but the ethanol often erases the labels on the tubes. Would that be OK to use liquid nitrogen instead? could it be too low of a temperature?
The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...
03 March 2021 4,107 4 View
How to accurately determine the total content of carbon in ammonia-ammonium carbonate mixed solution? When I am doing the experiment, I find it is difficult to accurately determine the total...
04 January 2021 2,694 1 View
Hello, I'm planning for my future research study on artificial salt lick. The study site that I've selected for this study has been confirmed with no presence of natural salt lick, thus I plan to...
02 August 2020 5,609 1 View
I'm currently using Nafion 211 for the proton exchange membrane in my microbial fuel cell experiment. I found lots of articles recommended to use Nafion 117 instead. My experiment was failed,...
11 November 2019 9,326 3 View
07 October 2019 8,027 3 View
Hi everyone, I'm currently working on bone-targeting polymeric nanoparticles for treatment of osteoporosis. Just wondering what kind of human osteoblast (or other bone cells) are best suitable for...
03 October 2019 9,077 2 View
Dear friends, i am a researcher on numerical computation, especially interested on PageRank computation. Now, I am teaching in a agriculture university, thus i want to do some applications to...
27 August 2019 7,598 4 View
Could anyone help me with staining T cells for surface activation markers (CD69) and Ki67/Granzyme B at the same time? The Ki67 staining protocol requires 70% ethanol treatment which might be too...
13 August 2019 7,513 4 View
I want to extract fungi and bacteria DNA from skin swab. Some articles used two 5mm steel beads per sample, some used 0.4-0.6mm ceramic beads per 0.5g sample. And other diameter for beads were...
05 August 2019 6,471 1 View
Recently, I want to use transwell assay to study glioma cells migration and invation. I have looked for a lot of protocols. And there were different opinions. I didn't find a complete and...
07 July 2019 1,175 3 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....
01 March 2021 3,325 2 View
The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...
28 February 2021 2,955 2 View
I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...
27 February 2021 8,128 1 View
I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...
23 February 2021 7,868 2 View
I need to synthesize MoS2 nanosheets by alternate methods without pure water as a solvent.
22 February 2021 9,246 1 View
I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?
21 February 2021 2,558 5 View
If ethanol has analgesic properties, why isn't it used for clinical purposes?
18 February 2021 8,227 4 View
I am currently preparing to isolate polyphenolics (Hydrolysable tannins) from aqueous extract of a plant. I want to set up a small sephadex column to guide the major isolation on a laerge...
18 February 2021 1,455 3 View