What are the most up-to-date methods for defining/mapping global land degradation and restoration?

I am seeking the best current methods and datasets (highest possible resolution) for defining and assessing global land degradation - ideally with a time series. I know there are different ways of...

24 November 2020 6,013 5 View

How do I write up thematic analysis results concisely?

Hello everyone, I've been writing up thematic analyses of interviews for the first time ever, and I can't help but write way too much for a thesis, let alone the papers that will make up a...

24 February 2020 5,044 11 View

Help With Autodock Vina error?

Hello, I've set up docking for vina and when i try to run the .txt file containing the inputs for docking i get this result: Parse error on line 7877 in file "protein.pdbqt": Unknown or...

28 January 2020 2,394 6 View

How to compare two experimental data sets with only a few overlapping individuals?

I have two experiments from the last two years in the field. The experiments are supposed to be the same, but different environmental conditions slightly skewed the exposed conditions relative to...

16 October 2017 9,864 3 View

Generating cDNA from low yield RNA?

25 April 2017 1,040 3 View

Labelling monoamines with dansyl chloride?

Hi, I'm trying to observe where dopamine localises at the cell surface of my cells of interest but fluorescent dopamine is very expensive to buy. I've read online that I should be able to label...

12 October 2016 5,404 2 View

GFP expression in cell-free systems - Why dasherGFP can be expressed just fine in a cell-free system, while cometGFP doesn't seem to work?

Can anyone think of a potential reason why dasherGFP can be expressed just fine in a cell-free system, while cometGFP doesn't seem to work? I'm not sure if it could be a translation problem or a...

22 February 2016 2,231 4 View

Programs to identify specific Protein-DNA interactions?

Are there any programs out there that can identify which specific DNA motif a transcription factor binds to, given the entire promoter sequence and TF sequence/structure. I've got the promoter...

12 February 2016 2,630 3 View

What is the best way to isolate and grow bacteriophages?

I'm working with Cronobacter sakazakii and attempting to isolate it from baby milk and use phages to inhibit growth.

26 November 2013 9,168 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

What kind of institutional environment do entrepreneurs need to achieve happiness?

Entrepreneurs are stressful, and happiness is a scarce resource. Happiness is crucial to business development.In addition to the work characteristics, individual characteristics have an effect on...

25 February 2021 876 2 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

Any interests in participating to an experiment in space?

Hello, The UN Office of Outer Space Affairs has a program called access to space for all that enables institutions to have hands on expertise related to access to space. There are three elements...

22 February 2021 7,980 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View