Would anyone ever have trouble revealing the Occludin protein in Immunohistochemistry?

05 May 2019 1 806 Report

Hi, I try to reveal Occludin in IHC but I do not get any signal. I am looking for a reliable protocol to reveal this protein on frozen coronal sections of rat brain.

Thank you

Atanaska Doncheva

If possible, choose an antibody that has been raised against the same species your sample is from.

The antibody may react with the same target protein from other species sharing sufficient amino acid sequence homology. If your sample is not from one of the species listed in the datasheet, this means that the species has not been tested and we cannot demonstrate suitability. A prediction of cross-reactivity is made based on sequence similarity.

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