Would anyone be able to suggest some papers or protocols to make frozen sections (cryostat sectioning) of zebrafish embryos (24hpf to 120hpf)?

03 March 2017 1 3K Report

We have been trying to section 120hpf fish and are having trouble with maintaining the integrity of the tissue. Also, we use hematoxylin and eosin stains.

Florian Siegerist

We have made great experience on zebrafish larvae with 2% PFA fixation and cryoprotection in 30% Sucrose in 1x PBS. We then infiltrate with OCT, snap freeze in liquid nitrogen and take sections at -20°C. We take sections ranging from 20µm - 4µm. Our protocol can be found here: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5333633/

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