Respected Ankit Srivastava, yes much work has been done on split gene. It started with the DNA study of adenoviruses. The It was found that the DNA sequences of these virus did not contain Polypeptides, and the sequence of DNA was split into exons and introns. I give you some sites where you may find the work of the scientists who worked upon this topic. Some of the scientists are Gilbert (1978), Lewin 1980). Weismen, 1980) however you may search these sites too for more www.annualreviews.org; www.science mag.org.