It measures a large number of parameters? I wonder which should be more important? Can anyone guide me about the most inter-linked (associated) traits?
The photosynthesis (A), stomatal conductance (gs), internal CO2 concentration (Ci) and transpiration (E) you can measure between 10:00 h and 12:00 h, on the penultimate leaves using a LI-6400 portable photosynthesis system (LI-COR Inc., Lincoln, NE, USA). Water use efficiency (WUE) and stomatal limitations to CO2 uptake you can also determine. then you can also calculate Water use efficiency. WUE is a ratio between A and E while stomatal limitations are the ratio between A and Ci.
I think it depends on the question you are trying to answer from the measurement. For eg. When i was working on heat and water stress in tomato, I took the data for net photosynthesis, stomatal conductance and internal carbon dioxide concentration.
The photosynthesis (A), stomatal conductance (gs), internal CO2 concentration (Ci) and transpiration (E) you can measure between 10:00 h and 12:00 h, on the penultimate leaves using a LI-6400 portable photosynthesis system (LI-COR Inc., Lincoln, NE, USA). Water use efficiency (WUE) and stomatal limitations to CO2 uptake you can also determine. then you can also calculate Water use efficiency. WUE is a ratio between A and E while stomatal limitations are the ratio between A and Ci.
The instrument is just an aid to record some data that can be helpful in giving answers to some questions. First devise the questions/objectives, then think which experiments can be developed to have answers to those questions, and definitely some precise data are to be recorded for that purpose. Here it is when you can think of using different aids/instruments for recording the desired data.
I think, as a PhD scholar, you need to investigate the adaptive/invasive attributes of parthenium under nutrient deficient, drought and saline environments. What makes this plant survives under abiotic stress/es, as well as what are physiological/biochemical basis of its competitiveness over other (native) species, and finally which comparative physiology enables Parthenium invade new areas (either virgin, drought prone, saline), and through replacement where others (native species) fail to survive, or are less competitive.
Thanks all. Yes, I am doing this for parthenium plants growing under moisture stress at the moment. I wanted to know the most appropriate parameters. The answer by Shah Fahad is helpful to determine those.
Dear Sir Abdul Khaliq, I agree with you and trying to work on these lines ....