If the sample is kept below 5 degree celsius temperature for 20 days. what wil be the effects on the TKN of waste water sample.?
Dear Piyush
TKN is a measure of oragnic-N + ammoniacal-N. Most raw wastewaters contain more organic-N than ammoniacal-N. Organic-N can mineralise to produce ammonical-N under warm conditions. If the samples are derived under tropical conditions, I doubt the microbes can mineralise organic-N below 5 oC. Therefore the answer is no effects.
Kind regards
Selva
Hallo Piyush,
TKN will be of course affected by the microorganisms found in the wastewater sample. However, the bio reaction of TKN will be slower at 5°C, which kann be even measured.
Anyway, you can minimize the change in TKN in two ways. Firstly you have to filter the sample via 0,45 µm paper filter before the storage. Secondly you have to acidify the sample to get low pH. These two actions will reduce the number of microorganisms and their activity in the sample, which let the changes in TKN at the lowest level during the 20 days storage.
Dear Piyush Kmr.
The ideal procedures have been duly explained by all Respondents.
1. Physical impurities may be filtered using simple filter paper.
The particles held in paper may be analysed.
2. If pH is below 6-7 level it is good.
3. Store at 5 deg C unstirred.
No appreciable chaange in TKN would be seen for 20 days .
Well wishes.
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