Can someone please help me to classify the image into alpha, beta and gamma haemolysis?

Dear all researchers, can you please help me to double confirm the result that I obtained in haemolytic test into alpha, beta and gamma group? This is the first time I performing this test and...

05 June 2016 1,063 21 View

Is there anyone who can provide the salting out protocol for butyrate extraction?

31 December 2015 1,956 8 View

Is there any titration method for vitamin A (retinol) detection?

10 November 2015 255 2 View

Does anyone kindly check for me regarding the following protocol for complexometric titration of Zn?

I'm not a chemist and does not know much about the theory behind the zinc titration method. I have a sample and wold like to test for the zinc content. Following is the protocol that I have...

09 October 2014 4,508 4 View

Why will contamination disappear when repeating a test for the same sample on the next day?

Dear researchers, Recently, I have been facing mysterious test results. I was collecting samples of probiotic cultures from a large fermenter tank into 100mL lab bottle under sterile conditions,...

09 October 2014 7,656 4 View

Do you think that zinc, iodine, Vitiman A, Vitamin D3 or Vitamin B2 can reduce the growing rate of probiotics?

Do you think that zinc, iodine, Vitiman A, Vitamin D3 or Vitamin B2 can reduce the growing rate of probiotics?

03 April 2014 5,067 2 View

Is there any drying method that can be used to replace spray drying?

May I know is there an alternative drying method that can be used to replace spray dryer? The sample used will be encapsulated bacterial cells.

02 March 2014 9,696 1 View

Can I autoclave the MRS medium with pH 1?

see above

02 March 2014 4,826 1 View

Why is the incubation time for probiotic cells to grow in milk shorter than in MRS broth?

I am wondering why the incubation time for probiotic cells to grow in milk is shorter than in MRS broth. According to the protocol, the bacteria strains need to culture in MRSB for 3 days before...

02 March 2014 9,839 3 View

Can anyone help me to check on the protocol of probiotic microencapsulation?

I did the experiment a number of times but am still not able to get the expected results. I would like to seek help from everyone. The protocol is as follows: 1. Dissolve maltodextrin and...

02 March 2014 6,229 12 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How can I find the thermophysical properties of high temperature microencapsulated phase change materials (MPCM)?

Hi everyone. I am looking for a high temperature MPCM. More specifically, I need the thermo physical properties of a microencapsulated phase change material with the melting temperature between...

14 February 2021 3,606 2 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View