Hallo,

I have done some Western Blot Experiments and now I am looking for a statistic test for my results. I want to see if the reduction of a transcriptionfactor has an impact on the expression of different proteins (reduction of protein). I have a wild type cell line as a control and I want to compare this with four different cell lines that have a reduction of the transcription factor.

I have analysed my Western Blot results with Image J and I have normalized them to my loading control (actin).

Is there a statistic test that can tell me if the reduction of a protein is significant in the four test lines compared to the wild type. I only found the anova test as a solution for my problem but I think this only shows me if there is a difference between all these groups and not only between the cell clones and the wild type.

Thanks for your help.