i study the genetic diversity of commiphora wightii so; which would be the best marker for profiling of genetic diverisity?
commiphora wightii (Guggal) species containing an bioactive compound named as guggulsterons which vary from genotype to genotype moreover the species showing wide genetic variability in nature.
Main reason they belongs to transcribe region and expected to be highly conserved. EST-SSRs may associate with genes and assumed to have some function (You can refer my two publications BMC Genomics, Mol.Bio.Evol).
Seems Commiphora wightii is an endangered and endemic medicinal plant around 81 EST seq.s are already submitted to NCBI GenBank. Refer them and you can use the available data. I believe sufficient literature is available.
Significance of Molecular Markers and DNA Based Technology in Research and Standardization of Medicinal Plants: A Review
http://www.ijorim.com/siteadmin/article_issue/14375609572_Bijoya_Chatterjee_Biochemistry%20.pdf.pdf
EST-SSR markers are derived from transcribed regions of the genome, they have higher rates of transferability than genomic SSRs. EST-SSR markers may help in
understanding these genetic relationships at a functional level
As Ramadevi said, EST SSRs belongs to transcribe region and expected to be highly conserved and you can easily get the sequence from NCBI data base instead of going for selective hybridization or sequencing for isolating the SSRs. Its easy way to get SSRs one click on search, download and design give for synthesis.
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