Cell wall hardening is of high significance in plant protections, particularly, fungus, lengthening shelf life, and tolerating stresses. We need to understand the mechanism of cell wall hardening. Thanks
In truth I don't know, and I am not sure I fully understand your question. What I do know is that pectins are acidic polysaccharides which join cell walls of adjacent cells together, like a glue, and that removing Ca with a metal chelator (EDTA), helped as needed by high temperature (65C), causes cells to separate from each other, the "glue" looses stickyness. This works very well with Arabidopsis leaf cells.