when we measure cytokines of infected cells we need to include heat kill. but why we do that. also both antibiotics killing bacteria and heat kill bacteria lead to release cytokines why?
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Heat killing is simpler because you don't have to worry about the effect of the residual antibiotics on your cells.
Difference between heat kill bacteria and antibiotic killing bacteria is that the heat killing is physical phenomenon where as use of antibiotics is a chemical phenomenon.Antibiotics may change physiological functions of the cells or enzymes.So, it stops the growth of organisms.
@Madhukar Baburao Deshmukh: All phenomena are natural (physical). "Chemical phenomenon" is physical phenomenon.
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