Shoxan Abdullah

4 Questions 3 Answers 0 Followers

Questions related from Shoxan Abdullah

Why my Western Blot running was not successful?

I run the western blot but the results not totally show anything only I sow the ladder. do that correlated with my primary antibody because proved that my secondary antibody worked alone with...

03 September 2020 4,721 9 View

What is the best protocol of western blot for protein detection and using PVDF membrane?

i am planing to look at alpha glycosides but I I have PVDF membrane and I will use semi-dry method but how I should do western blot and how prepare blocking buffer after running the membrane in...

28 August 2020 6,372 3 View

Why we need to include heat kill bacteria what is different between heat kill bacteria and antibiotic killing bacteria ?

when we measure cytokines of infected cells we need to include heat kill. but why we do that. also both antibiotics killing bacteria and heat kill bacteria lead to release cytokines why?

20 November 2017 4,452 4 View

What is the best concetration of LPS to induce THP-1 cells to get NLRP3 inflammasome ? in which concentraion cells die ?

if I have THP-1 cells what concentration of LPS need to use for induce the cells and get NLRP3 inflammasome? some article says 10ng/ml for 1*106 cells/ ml is this correct

14 September 2015 3,268 6 View