Why we mostly use sodium azide resistant Ecoli J53 as a recipient in conjugation experiments?

10 October 2017 0 5K Report

In many articles i saw that in conjugation experiments, mostly azide resistant Ecoli J53 is used.So what is the main reason behind this.

Badges
Science topic

More Waqas Salehzada's questions See All

Can anybody send me these Microbiology books in PDF form?

Hi i need these books in PDF form if anybody have kindly send me. 1. Microbiology for dummies by Jennifer Stearns. 2. Clinical microbiology made ridiculously easy 6th edition by Mark Gladwin. 3....

09 October 2018 4,631 0 View

What can be the reason for not getting mcr genes in colistin resistant bacteria?

What can be the possible reason for not getting mcr genes in colistin resistant bacteria? i checked colistin resistivity through broth micro dilution method and agar micro dilution method....

05 June 2018 3,263 7 View

In Conjugal transfer of plasmid mediated resistance genes isolated from chicken meat to human; what can be a suitable rationale?

Hi, i have some questions regarding my research work, hope someone can give me appropriate answer, I want to work on Conjugal transfer of plasmid mediated resistance genes of salmonela isolated...

09 October 2017 743 5 View

Question Regarding studying Conjugal Transfer of plasmid mediated resistance genes?

Hi, i have some questions regarding my research work, hope someone can give me appropriate answer, I want to work on Conjugal transfer of plasmid mediated resistance genes of salmonela isolated...

09 October 2017 3,463 0 View

What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View

Is it fare for editors of reputed Journals to put on hold manuscripts more than six months without review updates, Are they harassing researchers?

It is being seen that Editors of some reputed Journals are putting on hold the manuscripts and not updating review states even passing more than six months from submission. Researchers are...

24 July 2024 8,596 2 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

23 July 2024 6,783 1 View

What does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition of \lambda?

For a partition of an integer \lambda, what does the difference \lambda_j - \lambda_{j+1} represent in relation to the conjugate partition, , with \lambda_j being the j-th element of \lambda?

23 July 2024 8,008 1 View